Investigation of Neurogenesis in Kindled Wistar and Genetic Absence Epilepsy Rats

Objective: The most common type of epilepsy affecting about 50 million people worldwide is temporal lobe (TLE). Chemical and electrical kindling methods in animals can be used to form TLE model. In the present study, it was aimed investigate neurogenesis hippocampus adult kindled Wistar rats genetic absence from Strasbourg (GAERS) by immunofluorescence methods.
 
 Methods: Adult GAERS albino weighing 250-300 gr were injected pentylenetetrazole (PTZ) (35 mg/kg, s.c.) every other day produce chemical kindling. Animals having 5 times grade seizures considered kindled. Intracardiac perfusion performed under deep anesthesia on 7th 14th days after last seizure. Immunofluorescence demonstrate newly formed neurons, astroglial cells, mature using anti-doublecortin (DCX), anti-glial fibrillary acidic protein (GFAP), anti- neuronal nuclear antigen (NeuN) primary antibodies, respectively. Sections then examined a fluorescence microscope.
 Results: DCX (+) cells found increased control groups compared groups; PTZ groups. decreased their controls groups.
 Conclusion: findings study suggest that resistance reported previous studies might related this strain.